Small interfering RNA against CD86 during allergen challenge blocks experimental allergic asthma

Small interfering RNA against CD86 during allergen challenge blocks experimental allergic asthma

BACKGROUND CD86-CD28 interaction has been suggested as the principal costimulatory pathway for the activation and differentiation of naïve T cells in allergic inflammation. However, it remains uncertain whether this pathway also has an essential role in the effector phase. We sought to determine the contribution of CD86 on dendritic cells in the reactivation of allergen-specific Th2 cells. ME...

Small interfering RNA; principles, applications and challenges--

Gene silencing using RNAi (RNA interference), has recently been used as a successful laboratory technique in determining the function and control of gene expression and provides a wide range of applications in molecular biology and gene therapy. RNAi is a method of suppressing gene expression. In this direction, a single-stranded RNA molecule of about 21–23 nucleotides, called siRNA (small inte...

Allergen Avoidance in Allergic Asthma

Allergic asthma is the most frequent disease among the chronic respiratory disorders in pediatric age with an important social impact. In the last years, many efforts have been made to identify effective preventive approaches to get a better control of symptoms and to obtain the best future outcomes for the patients. In patients with allergic asthma triggered by the exposure to indoor allergens...

Induction of experimental allergic asthma in cat

19 cats were nper-sensitized to allergic asthma by receiving a concomitant spray ang injection of ovalbumin (10% fresh egg-white in normal saline) every other days for four weeks.then animals were anesthetized and left femoral artery cannulation performed to record atrerial pressure and take blood samples.left femoral vein was cannulated to infuse nutritional fluids and grugs as needed.trachea ...

Inflammation and Hyperreactivity in Mice Factor STAT6 Inhibits Allergic Airway Small Interfering RNA against Transcription